• The test contains about 180 multiple-choice questions, a number of which are grouped in sets toward the end of the test and based on descriptions of laboratory situations, diagrams, or experimental results.
• The content of the test is organized into three major areas: biochemistry, cell biology, and molecular biology and genetics. In addition to the total score, a subscore in each of these subfield areas is reported. Because these three disciplines are basic to the study of all organisms, test questions encompass both eukaryotes and prokaryotes.
• Throughout the test, there is an emphasis on questions requiring problem-solving skills (including mathematical calculations that do not require the use of a calculator) as well as content knowledge.
• While only two content areas in the following outline specifically mention methodology, questions on methodology and data interpretation are included in all sections.
• In developing questions for the test, the test development committee considers both the content of typical courses taken by undergraduates and the knowledge and abilities required for graduate work in the fields related to the test.
• Because of the diversity of undergraduate curricula, few examinees will have encountered all of the topics in the content outline. Consequently, no examinee should expect to be able to answer all questions on the edition of the test he or she takes.
• The three subscore areas are interrelated. Because of these interrelationships, individual questions or sets of questions may test more than one content area. Therefore, the relative emphases of the three areas in the following outline should not be considered definitive. Likewise, the topics listed are not intended to be all-inclusive but, rather, representative of the typical undergraduate experience.

I. BIOCHEMISTRY — 36%

A. Chemical and Physical Foundations
Thermodynamics and kinetics, Redox states, Water, pH, acid-base reactions, and buffers, Solutions and equilibria, Solute-solvent interactions , Chemical interactions and bonding, Chemical reaction mechanisms
B. Biomolecules: Structure, Assembly, Organization, and Dynamics
Small molecules, Macromolecules (for example, nucleic acids, polysaccharides,proteins, and complex Lipids), Supramolecular complexes (for example, membranes,ribosomes, and multienzyme complexes)
C. Catalysis and Binding
Enzyme reaction mechanisms and kinetics, Ligand-protein interaction (for example, hormone receptors,
substrates and effectors, transport proteins, and antigen-antibody interactions),
D. Major Metabolic Pathways
Carbon, nitrogen, and sulfur assimilation Anabolism, Catabolism,Synthesis and degradation of macromolecules,
E. Bioenergetics (including respiration and photosynthesis)
Energy transformations at the substrate level, Electron transport, Proton and chemical gradients
Energy coupling (phosphorylation and transport)
F. Regulation and Integration of Metabolism
Covalent modification of enzymes, Allosteric regulation, Compartmentation, Hormones
G. Methodology
Spectroscopy, Isotopes, Separation techniques (for example, centrifugation, chromatography, and electrophoresis), Immunotechniques

II. CELL BIOLOGY — 28%

A. Cellular Compartments of Prokaryotes and Eukaryotes: Organization, Dynamics, and Functions
Cellular membrane systems (structure and transport), Nucleus (envelope and matrix), Mitochondria and chloroplasts (including biogenesis and evolution)
B. Cell Surface and Communication
Extracellular matrix (including cell walls), Cell adhesion and junctions, Signal transduction, Receptor function, Excitable membrane systems
C. Cytoskeleton, Motility, and Shape
Actin-based systems (including muscle contraction), Microtubule-based systems, Intermediate filaments, Prokaryotic systems
D. Protein Synthesis and Processing
Regulation or translation, Posttranslational modification, Intracellular trafficking, Secretion and endocytosis,
E. Cell Division, Differentiation, and Development
Bacterial division, Meiosis and gametogenesis, Eukaryotic cell cycles, mitosis, and cytokinesis
Fertilization and early embryonic development (including positional information, homeotic genes, tissue-specific expression, nuclear and cytoplasmic interactions, growth factors and induction, environment, and polarity)

III. MOLECULAR BIOLOGY AND GENETICS — 36%

A. Genetic Foundations
Mendelian and non-Mendalian inheritance, Transformation, transduction, and conjugation, Recombination and complementation, Mutational analysis, Genetic mapping and linkage analysis,
B. Chromatin and Chromosomes
Karyotypes, Translocations, inversions, deletions, and duplications, Aneuploidy and polyploidy, Structure C. Genomics
Genome structure, Physical mapping, Repeated DNA and gene families, Gene identification, Transposable elements,
D. Genome Maintenance
DNA replication, DNA damage and repair, DNA modification, DNA recombination and gene conversion
E. Gene Expression
The genetic code, Transcription, RNA processing, Translation
F. Gene Regulation in Prokaryotes
Positive and negative control of the operon, Promoter recognition by RNA polymerases, Attenuation and antitermination,
G. Gene Regulation in Eukaryotes
Cis-acting regulatory elements, Trans-acting regulatory factors, Gene rearrangements and amplifications H. Bacteriophages and Animal and Plant Viruses
Genome replication and regulation, Virus assembly, Virus-host interactions.
I. Methodology
Restriction maps, Nucleic acid blotting and hybridization, DNA cloning in prokaryotes and eukaryotes
Sequencing and analysis, Protein-nucleic acid interaction